Top-Down and Middle-Down Analysis of Proteins with Post-Translational Modifications is now possible on your Q-ToF or Orbitrap QE mass spectrometer.
Our ExD Technology makes efficient Electron-Based Fragmentation possible, without affecting your instrument's performance and compatible with the fastest front-end separations including UPLC, Capillary Electrophoresis and Ion Mobility Separations.
Great Results come from a Small Package. Our ECD device is carefully engineered to fit in many tandem mass spectrometers. The ECD device sits in the ion flight path from the mass-resolving quad and the mass analyzer. A single pass through the ECD device takes microseconds and gives nearly complete sequence coverage of proteins even as large as carbonic anhydrase. Post-translational modifications such as phosphorylation are retained. Site-specific hydrogen/deuterium exchange (HDX) is feasible with middle and top-down analyses. In addition, side chain fragmentation allows for isobaric leucine and isoleucine residues to be distinguished to produce long sequence tags for de novo sequencing.