Select Publications Related to the ExD Cell
Publications
This publications page is an archive of peer-reviewed literature and scientific presentations directly related to our ExD Cell technology. e-MSion’s ExD Cell is the culmination of over a decade of academic research and commercial development.
Please explore our published and presented material for explanations of the technology and additional example data. As we publish new research, we will update our listings below.
Journal Articles
Chaturvedi, R.; Webb, I.K. Multiplexed Conformationally Selective, Localized Gas-Phase Hydrogen Deuterium Exchange of Protein Ions Enabled by Transmission-Mode Electron Capture Dissociation. Anal. Chemistry, 2022, 94 (25), 8975-8982, https://pubs.acs.org/doi/abs/10.1021/acs.analchem.2c00942
Cheung See Kit, M.; Carvalho, V.V.; Vilseck, J.Z.; Webb, I.K. Gas-phase ion/ion chemistry for structurally sensitive probes of gaseous protein ion structure: Electrostatic and electrostatic to covalent cross-linking. Int. J. Mass. Spectrom, 2021, 1387-3806. https://doi.org/10.1016/j.ijms.2021.116549.
Cheung See Kit, M.; Webb, I.K. Application of Multiple Length Cross-linkers to the Characterization of Gaseous Protein Structure. Anal. Chem. 2022 94 (39), 13301-13310, https://doi.org/10.1021/acs.analchem.2c03044
Dit Fouque, K.J.; Miller, S.A.; Pham, K.; Bhanu, N.V; Cintron-Diaz, Y.L.; Leyva, D.; Kaplan, D.; Voinov, V.G.; Ridgeway, M.E.; Park, M.A; Garcia, B.A.; Fernandez-Lima, F. Top-“Double-Down” Mass Spectrometry of Histone H4 Proteoforms: Tandem Ultraviolet-Photon and Mobility/Mass-Selected Electron Capture Dissociations. Anal. Chem. 2022, https://doi.org/10.1021/acs.analchem.2c03147
Greisch, J; den Boer, M.A.; Beurskens, F.; Schuurman, J.; Tamara, S.; Bondt, A.; Heck, A.J.R. Generating Informative Sequence Tags from Antigen-Binding Regions of Heavily Glycosylated IgA1 Antibodies by Native Top-Down Electron Capture Dissociation. JASMS, 2021, 32 (6), 1326-1335. https://pubs.acs.org/doi/10.1021/jasms.0c00461
J.D.; Fouque, K.J.D.; Santos-Fernandez, M.; Fernandez-Lima, F. A Bifunctional Leader Peptidase/ABC Transporter Protein Is Involved in the Maturation of the Lasso Peptide Cochonodin I from Streptococcus suis. J. Nat. Prod. 2021, 84 (10), 2683-2691. https://pubs.acs.org/doi/10.1021/acs.jnatprod.1c00514
Van Fossen, E. M.; Bednar, R. M.; Jana, S.; Franklin, R.; Beckman, J.; Karplus, P. A.; Mehl, R. A. Nanobody assemblies with fully flexible topology enabled by genetically encoded tetrazine amino acids. Science advances, 2022, 8(18) https://doi.org/10.1126/sciadv.abm6909
Beckman, J.S.; Voinov, V.G.; Hare, M.C.; Sturgeon, D.L.; Vasil’ev, Y.V.; Oppenheimer, D.M.; Shaw, J.B.; Wu, S.; Glaskin, R; Klein, C.; Schwarzer, C. ; Stafford, G. Improved Protein and PTM Characterization with a Practical Electron-Based Fragmentation on Q-TOF Instruments. JASMS, 2021, 32, 2081-2091, https://doi.org/10.1021/jasms.0c00482
Dit Fouque, K.J.; Kaplan, D.; Voinov, V.G.; Holck, F.H.V.; Jensen, O.N.; Fernandez-Lima, F. Proteoform Differentiation using Tandem Trapped Ion Mobility, Electron Capture Dissociation, and ToF Mass Spectrometry. Anal. Chemistry, 2021, 93, 9575-9582, https://doi.org/10.1021/acs.analchem.1c01735.
Dit Fouque, K.J.; Wellmann, M; Leyva Bombuse, D.; Santos-Fernandez, M; Cintron-Diaz, Y.L.; Gomez-Hernandez, M.E.; Kaplan, D.; Voinov, V.G.; Fernandez-Lima, F. Effective discrimination of gas-phase peptide conformers using TIMS-ECD-ToF MS/MS. Anal. Methods, 2021, https://doi.org/10.1039/D1AY01461G
Greisch, J.; den Boer, M.A.; Lai, S.; Gallagher, K.; Bondt, A.; Commandeur, J.; Heck, A.J.R. Extending Native Top-Down Electron Capture Dissociation to MDa Immunoglobulin Complexes Provides Useful Sequence Tags Covering Their Critical Variable Complementarity-Determining Regions. Anal. Chemistry, 2021, 93 (48), 16068-16075. https://doi.org/10.1021/acs.analchem.1c03740
Shen, X.; Xu, T.; Hakkila, B.; Hare, M.; Wang, Q.; Wang, Q.; Beckman, J.S.; Sun, L. Capillary Zone Electrophoresis-Electron-Capture Collision-Induced Dissociation on a Quadrupole Time-of-Flight Mass Spectrometer for Top-Down Characterization of Intact Proteins. JASMS, 2021, 32 (6), 1361-1369. https://doi.org/10.1021/jasms.0c00484
Shen, X.; Liang, Z.; Xu, T.; Yang, Z.; Wang, Q.; Chen, D.; Pham, L.; Du, W.; Sun, L. Investigating native capillary zone electrophoresis-mass spectrometry on a high-end quadrupole-time-of-flight mass spectrometer for the characterization of monoclonal antibodies. Int. J. Mass. Spectrom., 2021, 462, 116541. https://doi.org/10.1016/j.ijms.2021.116541
den Boer, M. A.; Greisch, J.-F.; Tamara, S.; Bondt, A.; Heck, A. J. R. Selectivity over Coverage in de Novo Sequencing of IgGs. Chem. Sci. 2020, 11 (43), 11886–11896.https://doi.org/10.1039/D0SC03438J.
Gadkari, V. V.; Ramírez, C. R.; Vallejo, D. D.; Kurulugama, R. T.; Fjeldsted, J. C.; Ruotolo, B. T. Enhanced Collision Induced Unfolding and Electron Capture Dissociation of Native-like Protein Ions. Anal. Chem. 2020, 92 (23), 15489–15496.https://doi.org/10.1021/acs.analchem.0c03372.
Shaw, J. B.; Liu, W.; Vasil′ev, Y. V.; Bracken, C. C.; Malhan, N.; Guthals, A.; Beckman, J. S.; Voinov, V. G. Direct Determination of Antibody Chain Pairing by Top-down and Middle-down Mass Spectrometry Using Electron Capture Dissociation and Ultraviolet Photodissociation. Anal. Chem. 2020, 92 (1), 766–773. https://doi.org/10.1021/acs.analchem.9b03129.
Vimer, S.; Ben-Nissan, G.; Morgenstern, D.; Kumar-Deshmukh, F.; Polkinghorn, C.; Quintyn, R. S.; Vasil’ev, Y. V.; Beckman, J. S.; Elad, N.; Wysocki, V. H.; Sharon, M. Comparative Structural Analysis of 20S Proteasome Ortholog Protein Complexes by Native Mass Spectrometry. ACS Cent. Sci. 2020, 6 (4), 573–588. https://doi.org/10.1021/acscentsci.0c00080.
Zhou, M.; Liu, W.; Shaw, J. B. Charge Movement and Structural Changes in the Gas-Phase Unfolding of Multimeric Protein Complexes Captured by Native Top-Down Mass Spectrometry. Anal. Chem. 2020, 92 (2), 1788–1795. https://doi.org/10.1021/acs.analchem.9b03469.
Shaw, J. B.; Malhan, N.; Vasil’ev, Y. V.; Lopez, N. I.; Makarov, A.; Beckman, J. S.; Voinov, V. G. Sequencing Grade Tandem Mass Spectrometryfor Top–Down Proteomics Using Hybrid Electron Capture Dissociation Methods in a Benchtop Orbitrap Mass Spectrometer. Chem. 2018, 90 (18), 10819–10827. https://doi.org/10.1021/acs.analchem.8b01901.
Fort, K. L.; Cramer, C. N.; Voinov, V. G.; Vasil’ev, Y. V.; Lopez, N. I.; Beckman, J. S.; Heck, A. J. R. Exploring ECD on a Benchtop Q Exactive Orbitrap Mass Spectrometer. Journal of Proteome Research, 2018, 17 (2), 926–933. https://doi.org/10.1021/acs.jproteome.7b00622.
Mukherjee, S.; Kapp, E. A.; Lothian, A.; Roberts, A. M.; Vasil’ev, Y. V.; Boughton, B. A.; Barnham, K. J.; Kok, W. M.; Hutton, C. A.; Masters, C. L.; et al. Characterization and Identification of Dityrosine Cross-Linked Peptides Using Tandem Mass Spectrometry. Analytical Chemistry. 2017, 89 (11), 6136–6145. https://doi.org/10.1021/acs.analchem.7b00941.
Voinov, V. G.; Bennett, S. E.; Barofsky, D. F. Electron-Induced Dissociation of Peptides in a Triple Quadrupole Mass Spectrometer Retrofitted with an Electromagnetostatic Cell. Am. Soc. Mass Spectrom. 2015, 26 (5), 752–761. https://doi.org/10.1007/s13361-014-1074-x.
Voinov, V. G.; Hoffman, P. D.; Bennett, S. E.; Beckman, J. S.; Barofsky, D. F. Electron Capture Dissociation of Sodium-Adducted Peptides on a Modified Quadrupole/Time-of-Flight Mass Spectrometer. Journal of The American Society for Mass Spectrometry, 2015, 26 (12), 2096–2104. https://doi.org/10.1007/s13361-015-1230-y.
Voinov, V. G.; Bennett, S. E.; Beckman, J. S.; Barofsky, D. F. ECD of Tyrosine Phosphorylation in a Triple Quadrupole Mass Spectrometer with a Radio-Frequency-Free Electromagnetostatic Cell. Am. Soc. Mass Spectrom. 2014, 25 (10), 1730–1738. https://doi.org/10.1007/s13361-014-0956-2.
Voinov, V. G.; Deinzer, M. L.; Beckman, J. S.; Barofsky, D. F. Electron Capture, Collision-Induced, and Electron Capture-Collision Induced Dissociation in Q-TOF. J Am Soc Mass Spectrom, 2011, 22 (4), 607–611. https://doi.org/10.1007/s13361-010-0072-x.
Voinov, V. G.; Beckman, J. S.; Deinzer, M. L.; Barofsky, D. F. Electron-Capture Dissociation (ECD), Collision-Induced Dissociation (CID) and ECD/CID in a Linear Radio-Frequency-Free Magnetic Cell. Rapid Commun Mass Spectrom, 2009, 23 (18), 3028–3030. https://doi.org/10.1002/rcm.4209.
Voinov, V. G.; Deinzer, M. L.; Barofsky, D. F. Radio-Frequency-Free Cell for Electron Capture Dissociation in Tandem Mass Spectrometry. Anal Chem. 2009, 81 (3), 1238–1243. https://doi.org/10.1021/ac802084w.
Voinov, V. G.; Deinzer, M. L.; Barofsky, D. F. Electron Capture Dissociation in a Linear Radiofrequency-Free Magnetic Cell. Rapid Commun Mass Spectrom, 2008, 22 (19), 3087–3088. https://doi.org/10.1002/rcm.3709.
Posters
Vasil’ev, Y. V.; Lopez, N. I.; Voinov, V. G.; Beckman, J. S.; Knierman, M.; Klein, C.; Newton, K.; Kurulugama, R.; Stafford, G.; Fjeldsted, J. ECD and EID fragmentation of peptides and intact proteins using a Quadrupole Time-of-Flight Mass Spectrometer. Poster presented at: 66th Annual ASMS Conference on Mass Spectrometry and Allied Topics. 2018. San Diego CA
Vasil’ev, Y. V.; Voinov, V. G.; Malhan, N.; Lopez, N. I.; Beckman, J. S.; Shaw, J. B. Hybrid ECD methods for middle-down and top-down proteomics implemented in a benchtop quadrupole-Orbitrap mass spectrometer. Poster presented at: 66th Annual ASMS Conference on Mass Spectrometry and Allied Topics. 2018. San Diego CA.
Vasil’ev, Y. V.; Lopez, N. I.; Voinov, V. G.; Beckman, J. S.; Hydrogen Deuterium Exchange of Native Ubiquitin Probed by ECD with Complete Sequence Coverage. Poster presented at: 65th Annual ASMS Conference on Mass Spectrometry and Allied Topics. 2017. Indianapolis IN.
Vasil’ev, Y. V.; Lopez, N. I.; Voinov, V. G.; Beckman, J. S.; Barofsky, D. F. Complete sequence coverage via ECD in a Q-ToF versus ETD/CID/HCD in an Orbitrap of 6+ and 7+ protonated ubiquitin. Poster presented at: 64th Annual ASMS Conference on Mass Spectrometry and Allied Topics. 2016. San Antonio TX.
Voinov, V. G.; Vasil’ev, Y. V.; Beckman, J. S.; Barofsky, D. F. ECD Analysis of Serine Phosphorylated Peptides using EMS Cell. Poster presented at: 64th Annual ASMS Conference on Mass Spectrometry and Allied Topics. 2016. San Antonio TX.
Voinov, V. G.; Bennett, S. E.; Hoffman, P. D.; Beckman, J. S.; Barofsky, D. F. Electron Capture Dissociation of Sodium-Adducted Peptides on a Modified SCIEX Q-Star Q-TOF Mass Spectrometer. Poster presented at: 62nd Annual ASMS Conference on Mass Spectrometry and Allied Topics. 2014. Baltimore MD.
Voinov, V. G.; Lin, Y. C.; Edge, A.; Bennett, S. E.; Beckman, J. S.; Barofsky, D. F. ECD in an RF-Free Electromagnetostatic Cell on a Triple Quadrupole Mass Spectrometer. Poster presented at: 19th International Mass Spectrometry Conference. 2012. Kyoto, Japan.