Select Publications Related to the ExD Cell

Publications

This publications page is an archive of peer-reviewed literature and scientific presentations directly related to our ExD Cell technology. e-MSion’s ExD Cell is the culmination of over a decade of academic research and commercial development.

Please explore our published and presented material for explanations of the technology and additional example data. As we publish new research, we will update our listings below.

Journal Articles

Chaturvedi, R.; Webb, I.K. Multiplexed Conformationally Selective, Localized Gas-Phase Hydrogen Deuterium Exchange of Protein Ions Enabled by Transmission-Mode Electron Capture Dissociation. Anal. Chemistry, 2022, 94 (25), 8975-8982, https://pubs.acs.org/doi/abs/10.1021/acs.analchem.2c00942

Cheung See Kit, M.; Carvalho, V.V.; Vilseck, J.Z.; Webb, I.K. Gas-phase ion/ion chemistry for structurally sensitive probes of gaseous protein ion structure: Electrostatic and electrostatic to covalent cross-linking. Int. J. Mass. Spectrom, 2021, 1387-3806. https://doi.org/10.1016/j.ijms.2021.116549.

Cheung See Kit, M.; Webb, I.K. Application of Multiple Length Cross-linkers to the Characterization of Gaseous Protein Structure. Anal. Chem. 2022 94 (39), 13301-13310, https://doi.org/10.1021/acs.analchem.2c03044

Dit Fouque, K.J.; Miller, S.A.; Pham, K.; Bhanu, N.V; Cintron-Diaz, Y.L.; Leyva, D.; Kaplan, D.; Voinov, V.G.; Ridgeway, M.E.; Park, M.A; Garcia, B.A.; Fernandez-Lima, F. Top-“Double-Down” Mass Spectrometry of Histone H4 Proteoforms: Tandem Ultraviolet-Photon and Mobility/Mass-Selected Electron Capture Dissociations. Anal. Chem. 2022, https://doi.org/10.1021/acs.analchem.2c03147

Greisch, J; den Boer, M.A.; Beurskens, F.; Schuurman, J.; Tamara, S.; Bondt, A.; Heck, A.J.R. Generating Informative Sequence Tags from Antigen-Binding Regions of Heavily Glycosylated IgA1 Antibodies by Native Top-Down Electron Capture Dissociation. JASMS, 2021, 32 (6), 1326-1335. https://pubs.acs.org/doi/10.1021/jasms.0c00461

J.D.; Fouque, K.J.D.; Santos-Fernandez, M.; Fernandez-Lima, F. A Bifunctional Leader Peptidase/ABC Transporter Protein Is Involved in the Maturation of the Lasso Peptide Cochonodin I from Streptococcus suis. J. Nat. Prod. 2021, 84 (10), 2683-2691. https://pubs.acs.org/doi/10.1021/acs.jnatprod.1c00514

Shaw, J.B.; Cooper-Shepherd, D.A; Hewitt, D. Wildgoose, J.L.; Beckman, J.S.; Langridge, J.I.; Voinov, V.G. Enhanced Top-Down Protein Characterization with Electron Capture Dissociation and Cyclic Ion Mobility Spectrometry. Anal. Chem. 2022, 94, 9, 3888–3896, https://doi.org/10.1021/acs.analchem.1c04870

Van Fossen, E. M.; Bednar, R. M.; Jana, S.; Franklin, R.; Beckman, J.; Karplus, P. A.; Mehl, R. A.  Nanobody assemblies with fully flexible topology enabled by genetically encoded tetrazine amino acids. Science advances, 2022, 8(18) https://doi.org/10.1126/sciadv.abm6909

Beckman, J.S.; Voinov, V.G.; Hare, M.C.; Sturgeon, D.L.; Vasil’ev, Y.V.; Oppenheimer, D.M.; Shaw, J.B.; Wu, S.; Glaskin, R; Klein, C.; Schwarzer, C. ; Stafford, G. Improved Protein and PTM Characterization with a Practical Electron-Based Fragmentation on Q-TOF Instruments. JASMS, 2021, 32, 2081-2091, https://doi.org/10.1021/jasms.0c00482

Dit Fouque, K.J.; Kaplan, D.; Voinov, V.G.; Holck, F.H.V.; Jensen, O.N.; Fernandez-Lima, F. Proteoform Differentiation using Tandem Trapped Ion Mobility, Electron Capture Dissociation, and ToF Mass Spectrometry. Anal. Chemistry, 2021, 93, 9575-9582, https://doi.org/10.1021/acs.analchem.1c01735.

Dit Fouque, K.J.; Wellmann, M; Leyva Bombuse, D.; Santos-Fernandez, M; Cintron-Diaz, Y.L.; Gomez-Hernandez, M.E.; Kaplan, D.; Voinov, V.G.; Fernandez-Lima, F. Effective discrimination of gas-phase peptide conformers using TIMS-ECD-ToF MS/MS. Anal. Methods, 2021, https://doi.org/10.1039/D1AY01461G

Greisch, J.; den Boer, M.A.; Lai, S.; Gallagher, K.; Bondt, A.; Commandeur, J.; Heck, A.J.R. Extending Native Top-Down Electron Capture Dissociation to MDa Immunoglobulin Complexes Provides Useful Sequence Tags Covering Their Critical Variable Complementarity-Determining Regions. Anal. Chemistry, 2021, 93 (48), 16068-16075. https://doi.org/10.1021/acs.analchem.1c03740

Shen, X.; Xu, T.; Hakkila, B.; Hare, M.; Wang, Q.; Wang, Q.; Beckman, J.S.; Sun, L. Capillary Zone Electrophoresis-Electron-Capture Collision-Induced Dissociation on a Quadrupole Time-of-Flight Mass Spectrometer for Top-Down Characterization of Intact Proteins. JASMS, 2021, 32 (6), 1361-1369. https://doi.org/10.1021/jasms.0c00484

Shen, X.; Liang, Z.; Xu, T.; Yang, Z.; Wang, Q.; Chen, D.; Pham, L.; Du, W.; Sun, L. Investigating native capillary zone electrophoresis-mass spectrometry on a high-end quadrupole-time-of-flight mass spectrometer for the characterization of monoclonal antibodies. Int. J. Mass. Spectrom., 2021, 462, 116541. https://doi.org/10.1016/j.ijms.2021.116541

den Boer, M. A.; Greisch, J.-F.; Tamara, S.; Bondt, A.; Heck, A. J. R. Selectivity over Coverage in de Novo Sequencing of IgGs. Chem. Sci. 2020, 11 (43), 11886–11896.https://doi.org/10.1039/D0SC03438J.

Gadkari, V. V.; Ramírez, C. R.; Vallejo, D. D.; Kurulugama, R. T.; Fjeldsted, J. C.; Ruotolo, B. T. Enhanced Collision Induced Unfolding and Electron Capture Dissociation of Native-like Protein Ions. Anal. Chem. 2020, 92 (23), 15489–15496.https://doi.org/10.1021/acs.analchem.0c03372.

Shaw, J. B.; Liu, W.; Vasil′ev, Y. V.; Bracken, C. C.; Malhan, N.; Guthals, A.; Beckman, J. S.; Voinov, V. G. Direct Determination of Antibody Chain Pairing by Top-down and Middle-down Mass Spectrometry Using Electron Capture Dissociation and Ultraviolet Photodissociation. Anal. Chem. 2020, 92 (1), 766–773. https://doi.org/10.1021/acs.analchem.9b03129.

Vimer, S.; Ben-Nissan, G.; Morgenstern, D.; Kumar-Deshmukh, F.; Polkinghorn, C.; Quintyn, R. S.; Vasil’ev, Y. V.; Beckman, J. S.; Elad, N.; Wysocki, V. H.; Sharon, M. Comparative Structural Analysis of 20S Proteasome Ortholog Protein Complexes by Native Mass Spectrometry. ACS Cent. Sci. 2020, 6 (4), 573–588. https://doi.org/10.1021/acscentsci.0c00080.

Williams, J. P.; Morrison, L. J.; Brown, J. M.; Beckman, J. S.; Voinov, V. G.; Lermyte, F. Top-Down Characterization of Denatured Proteins and Native Protein Complexes Using Electron Capture Dissociation Implemented within a Modified Ion Mobility-Mass Spectrometer. Anal. Chem. 2020, 92 (5), 3674–3681. https://doi.org/10.1021/acs.analchem.9b04763.

Zhou, M.; Liu, W.; Shaw, J. B. Charge Movement and Structural Changes in the Gas-Phase Unfolding of Multimeric Protein Complexes Captured by Native Top-Down Mass Spectrometry. Anal. Chem. 2020, 92 (2), 1788–1795. https://doi.org/10.1021/acs.analchem.9b03469.

Shaw, J. B.; Malhan, N.; Vasil’ev, Y. V.; Lopez, N. I.; Makarov, A.; Beckman, J. S.; Voinov, V. G. Sequencing Grade Tandem Mass Spectrometryfor Top–Down Proteomics Using Hybrid Electron Capture Dissociation Methods in a Benchtop Orbitrap Mass Spectrometer. Chem. 2018, 90 (18), 10819–10827. https://doi.org/10.1021/acs.analchem.8b01901.

Fort, K. L.; Cramer, C. N.; Voinov, V. G.; Vasil’ev, Y. V.; Lopez, N. I.; Beckman, J. S.; Heck, A. J. R. Exploring ECD on a Benchtop Q Exactive Orbitrap Mass Spectrometer. Journal of Proteome Research, 2018, 17 (2), 926–933. https://doi.org/10.1021/acs.jproteome.7b00622.

Mukherjee, S.; Kapp, E. A.; Lothian, A.; Roberts, A. M.; Vasil’ev, Y. V.; Boughton, B. A.; Barnham, K. J.; Kok, W. M.; Hutton, C. A.; Masters, C. L.; et al. Characterization and Identification of Dityrosine Cross-Linked Peptides Using Tandem Mass Spectrometry. Analytical Chemistry. 2017, 89 (11), 6136–6145. https://doi.org/10.1021/acs.analchem.7b00941.

Voinov, V. G.; Bennett, S. E.; Barofsky, D. F. Electron-Induced Dissociation of Peptides in a Triple Quadrupole Mass Spectrometer Retrofitted with an Electromagnetostatic Cell. Am. Soc. Mass Spectrom. 2015, 26 (5), 752–761. https://doi.org/10.1007/s13361-014-1074-x.

Voinov, V. G.; Hoffman, P. D.; Bennett, S. E.; Beckman, J. S.; Barofsky, D. F. Electron Capture Dissociation of Sodium-Adducted Peptides on a Modified Quadrupole/Time-of-Flight Mass Spectrometer. Journal of The American Society for Mass Spectrometry, 2015, 26 (12), 2096–2104. https://doi.org/10.1007/s13361-015-1230-y.

Voinov, V. G.; Bennett, S. E.; Beckman, J. S.; Barofsky, D. F. ECD of Tyrosine Phosphorylation in a Triple Quadrupole Mass Spectrometer with a Radio-Frequency-Free Electromagnetostatic Cell. Am. Soc. Mass Spectrom. 2014, 25 (10), 1730–1738. https://doi.org/10.1007/s13361-014-0956-2.

Voinov, V. G.; Deinzer, M. L.; Beckman, J. S.; Barofsky, D. F. Electron Capture, Collision-Induced, and Electron Capture-Collision Induced Dissociation in Q-TOF. J Am Soc Mass Spectrom, 2011, 22 (4), 607–611. https://doi.org/10.1007/s13361-010-0072-x.

Voinov, V. G.; Beckman, J. S.; Deinzer, M. L.; Barofsky, D. F. Electron-Capture Dissociation (ECD), Collision-Induced Dissociation (CID) and ECD/CID in a Linear Radio-Frequency-Free Magnetic Cell. Rapid Commun Mass Spectrom, 2009, 23 (18), 3028–3030. https://doi.org/10.1002/rcm.4209.

Voinov, V. G.; Deinzer, M. L.; Barofsky, D. F. Radio-Frequency-Free Cell for Electron Capture Dissociation in Tandem Mass Spectrometry. Anal Chem. 2009, 81 (3), 1238–1243. https://doi.org/10.1021/ac802084w.

Voinov, V. G.; Deinzer, M. L.; Barofsky, D. F. Electron Capture Dissociation in a Linear Radiofrequency-Free Magnetic Cell. Rapid Commun Mass Spectrom, 2008, 22 (19), 3087–3088. https://doi.org/10.1002/rcm.3709.

Posters

Beckman, J. S.; Voinov, V. G.; Vasil’ev, Y. V.; Williams, J.; Brown, J.; Shaw, B. Efficient top-down characterization of native proteoforms by ECD at chromatographic speeds with Q-ToF, Orbitrap and IMS Instruments. Poster presented at: 1st European Top-Down Proteomics Symposium. 2019. Paris, France.
Beckman, J. S.; Voinov, V. G.; Roberts, B.R. Facile Electron Capture Dissociation on Q-ToFs and Orbitraps for top-down proteomics at chromatographic time scales. Poster presented at: 21st International Mass Spectrometry Conference. 2018. Florence, Italy

Vasil’ev, Y. V.; Lopez, N. I.; Voinov, V. G.; Beckman, J. S.; Knierman, M.; Klein, C.; Newton, K.; Kurulugama, R.; Stafford, G.; Fjeldsted, J. ECD and EID fragmentation of peptides and intact proteins using a Quadrupole Time-of-Flight Mass Spectrometer. Poster presented at: 66th Annual ASMS Conference on Mass Spectrometry and Allied Topics. 2018. San Diego CA

Vasil’ev, Y. V.; Voinov, V. G.; Malhan, N.;  Lopez, N. I.; Beckman, J. S.; Shaw, J. B. Hybrid ECD methods for middle-down and top-down proteomics implemented in a benchtop quadrupole-Orbitrap mass spectrometer. Poster presented at: 66th Annual ASMS Conference on Mass Spectrometry and Allied Topics. 2018. San Diego CA.
Fort, K. L.; Heck, A. J. R.; Voinov, V. G.; Vasil’ev, Y. V.; Beckman, J. S.; ECD in an RF-Free Electromagnetostatic Cell on a High Resolution/High Throughput Q-Exactive Orbitrap Mass Spectrometer. Poster presented at: 65th Annual ASMS Conference on Mass Spectrometry and Allied Topics. 2017. Indianapolis IN.

Vasil’ev, Y. V.;  Lopez, N. I.; Voinov, V. G.; Beckman, J. S.; Hydrogen Deuterium Exchange of Native Ubiquitin Probed by ECD with Complete Sequence Coverage. Poster presented at: 65th Annual ASMS Conference on Mass Spectrometry and Allied Topics. 2017. Indianapolis IN.
Beckman, J. S.; Vasil’ev, Y. V.; Lopez, N. I.; Voinov, V. G.; Top-Down Sequence-specific Copper and Zinc Retention from ECD and ETD of Superoxide Dismutase for ALS Spinal cord. Poster presented at: 64th Annual ASMS Conference on Mass Spectrometry and Allied Topics. 2016. San Antonio TX.

Vasil’ev, Y. V.; Lopez, N. I.; Voinov, V. G.; Beckman, J. S.; Barofsky, D. F. Complete sequence coverage via ECD in a Q-ToF versus ETD/CID/HCD in an Orbitrap of 6+ and 7+ protonated ubiquitin. Poster presented at: 64th Annual ASMS Conference on Mass Spectrometry and Allied Topics. 2016. San Antonio TX.

Voinov, V. G.; Vasil’ev, Y. V.; Beckman, J. S.; Barofsky, D. F. ECD Analysis of Serine Phosphorylated Peptides using EMS Cell. Poster presented at: 64th Annual ASMS Conference on Mass Spectrometry and Allied Topics. 2016. San Antonio TX.
Vasil’ev, Y. V.; Voinov, V. G.; Beckman, J. S.; Barofsky, D. F. Top-Down Electron-Capture Dissociation of Proteins in a QTOF Mass Spectrometer. Poster presented at: 63rd Annual ASMS Conference on Mass Spectrometry and Allied Topics. 2015. St. Louis MO.
Newton, K.; Stafford, G.; Voinov, V. G.; Bennett, S. E.; Hoffman, P. D.; Beckman, J. S.; Huang, L.; Maier, C. S.; Barofsky, D. F. ECD, ECD/CID and HPLC ECD MRM of Phosphorylated Peptides in a Triple Quadrupole Mass Spectrometer. Poster presented at: 62nd Annual ASMS Conference on Mass Spectrometry and Allied Topics. 2014. Baltimore MD.

Voinov, V. G.; Bennett, S. E.; Hoffman, P. D.; Beckman, J. S.; Barofsky, D. F. Electron Capture Dissociation of Sodium-Adducted Peptides on a Modified SCIEX Q-Star Q-TOF Mass Spectrometer. Poster presented at: 62nd Annual ASMS Conference on Mass Spectrometry and Allied Topics. 2014. Baltimore MD.
Voinov, V. G.; Lin, Y. C.; Bennett, S. E.; Beckman, J. S.; Barofsky, D. F. ECD in an RF-Free Electromagnetostatic Cell on a Triple Quadrupole Mass Spectrometer. Poster presented at: 60th Annual ASMS Conference on Mass Spectrometry and Allied Topics. 2012. Vancouver BC.

Voinov, V. G.; Lin, Y. C.; Edge, A.; Bennett, S. E.; Beckman, J. S.; Barofsky, D. F. ECD in an RF-Free Electromagnetostatic Cell on a Triple Quadrupole Mass Spectrometer. Poster presented at: 19th International Mass Spectrometry Conference. 2012. Kyoto, Japan.
Voinov, V. G.; Chen, L.; Beckman, J. S.; Deinzer, M. L.; Park, M.; Knudsen, T.; Barofsky, D. F. ECD in an Electromagnetostatic Cell on a Quadrupole/Time-of-Flight Mass Spectrometer. Poster presented at: 58th Annual ASMS Conference on Mass Spectrometry and Allied Topics. 2010. Salt Lake City UT.
Voinov, V. G.; Beckman, J. S.; Deinzer, M. L.; Barofsky, D. F. RF-Free EMS Cell for ECD, CID and Combined ECD/CID Mass Spectrometry. Poster presented at: 57th Annual ASMS Conference on Mass Spectrometry and Allied Topics. 2009. Philadelphia PA.
Voinov, V. G.; Deinzer, M. L.; Barofsky, D. F. ECD in a Linear RF-Free Magnetic Cell. Poster presented at: 56th Annual ASMS Conference on Mass Spectrometry and Allied Topics. 2008. Denver CO.