The e-MSion ExD cell makes it possible to enable fast and effective electron-capture dissociation (ECD) of peptide bonds, which can substantially improve the bottom up as well as top down characterization of protein proteoforms. The ExD cell can be retrofitted into current generations of the most widely used types mass spectrometers including Q-TOFs and Orbitrap QEs without changing the flight of analyte ions through the cell. Because of this, the installation of the ExD cell maintains the key functionality of the basic mass spectrometer without significantly altering transmission, sensitivity or resolution.
CID fragmentation remains fully functional and provides complimentary fragmentation information when combined with ECD to provide more confidence in peptide and protein identification. The ExD cell also can produce side chain fragmentation (particularly w ions) that can distinguish isobaric leucine from isoleucine and aspartate from isoaspartate. Because side chain fragmentation is more commonly produced by higher energy electrons from Electron-Induced Dissociation, we have adopted the more general term ExD to describe the cell. To learn more about applications of the ExD Cell, visit our Example Data and Publications pages