Click the toggles below to see detailed figures and captions describing some ExD example data.
Phosphorylation Site Localization
Figure 2 – Top: ECD product ion mass spectrum of the [M+20H]20+ precursor of the serine phosphorylated protein alpha-casein, acquired using the ExD Cell in a Thermo Fisher Scientific Orbitrap Q Exactive. Sequence coverage was calculated by ProSight Lite to be 35%, using centroids with S:N > 2 and mass error < 10 ppm. Single-residue-resolution evidence confirmed the presence of two out of the eight phosphoserine residues. Middle & Bottom: Magnified regions showing evidence of phosphorylation at the 46th and 48th residues, respectively.
Figure 3 – ECD product ion mass spectrum of the serine phosphorylated, lysine acetylated Biotin-labeled Histone H3 peptide (residues 1-21), acquired using the ExD Cell in a Thermo Fisher Scientific Orbitrap Q Exactive. The mass range 1700-3000 has been magnified 25X to emphasize the z15-z17 fragment ions indicating the presence of phosphorylated serine.
Disulfide Bond Localization
See Fort et al. J Proteome Res 17:926-933 (2018) for data regarding selective disulfide dissociation.
Top-Down Protein Sequencing
See Shaw et al. Anal Chem 90(18):10819-10827 (2018) for data regarding the use of multiple complementary fragmentation techniques in an Orbitrap QE Plus to sequence antibody molecules in a top-down workflow.