THERMO FISHER SCIENTIFIC

ExD for Thermo Orbitraps and Hybrid Orbitraps

Thermo Fisher Orbitrap

The Orbitrap-model ExD Cell is in the Early Adopter product phase. Please contact e-MSion for details, or to inquire about becoming an Early Adopter.

Features

  • Easy swap-in/out installation

  • Enables electron-based fragmentation for Orbitrap and Hybrid Orbitrap benchtop mass spectrometers

Figure 1

Diagram of the Thermo Fisher Scientific Orbitrap QE, depicting where in the context of the ion optics the ExD Cell is installed.
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Figure 2

A digital cutaway rendering of the Orbitrap-model ExD Cell.

Figure 3

A photograph of the Orbitrap-model ExD Cell.

Figure 4

The ExD Cell installed in a Thermo Fisher Scientific Orbitrap QE. The C-Trap was temporarily removed for installation.

Example Data

Click the toggles below to see detailed figures and captions describing some ExD example data.

Phosphorylation Site Localization

 

Figure 2 – Top: ECD product ion mass spectrum of the [M+20H]20+ precursor of the serine phosphorylated protein alpha-casein, acquired using the ExD Cell in a Thermo Fisher Scientific Orbitrap Q Exactive. Sequence coverage was calculated by ProSight Lite to be 35%, using centroids with S:N > 2 and mass error < 10 ppm. Single-residue-resolution evidence confirmed the presence of two out of the eight phosphoserine residues. Middle & Bottom: Magnified regions showing evidence of phosphorylation at the 46th and 48th residues, respectively.

 

Figure 3 – ECD product ion mass spectrum of the serine phosphorylated, lysine acetylated Biotin-labeled Histone H3 peptide (residues 1-21), acquired using the ExD Cell in a Thermo Fisher Scientific Orbitrap Q Exactive. The mass range 1700-3000 has been magnified 25X to emphasize the z15-z17 fragment ions indicating the presence of phosphorylated serine.

 

Disulfide Bond Localization

See Fort et al. J Proteome Res 17:926-933 (2018) for data regarding selective disulfide dissociation.

Top-Down Protein Sequencing

See Shaw et al. Anal Chem 90(18):10819-10827 (2018) for data regarding the use of multiple complementary fragmentation techniques in an Orbitrap QE Plus to sequence antibody molecules in a top-down workflow.

Visit our Publications page to view more published and presented data.