Instrument: Waters SELECT SERIES Cyclic IMS
Summary: Post-cIMS ECD of Trastuzumab heavy chain peptide shows IM separation of Asp and isoAsp containing peptides. Characteristic ECD z-57 and c+57 fragment ions confirm presence of isoaspartate.
Instrument: Agilent 6545XT AdvanceBio LC/Q-TOF
Summary: W-ions from MS/MS ECD differentiate isobaric leu/ile residues in peptide REALLYisoDELIGHTFLK.
Instrument: Thermo Scientific Q Exactive Orbitrap
Summary: Native top-down characterization of intact monoclonal antibody. ECD yields disulfide bond cleavage to liberate the intact light chain as well as sequencing of the CDR3 region of both chains.
Instrument: Agilent 6545XT AdvanceBio LC/Q-TOF
Summary: Localizing glycosite by LC-MS/MS ECD of infliximab LysC digest
Instrument: Thermo Scientific Q Exactive Orbitrap
Summary: 89% sequence coverage from direct infusion of denatured carbonic.
