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ExDViewer File Directory
Isobar Differentiation

Instrument: Waters SELECT SERIES Cyclic IMS

Summary: Post-cIMS ECD of Trastuzumab heavy chain peptide shows IM separation of Asp and isoAsp containing peptides. Characteristic ECD z-57 and c+57 fragment ions confirm presence of isoaspartate.

Instrument: Agilent 6545XT AdvanceBio LC/Q-TOF

Summary: W-ions from MS/MS ECD differentiate isobaric leu/ile residues in peptide REALLYisoDELIGHTFLK.

Top-Down Characterization

Instrument: Thermo Scientific Q Exactive Orbitrap

Summary: Native top-down characterization of intact monoclonal antibody. ECD yields disulfide bond cleavage to liberate the intact light chain as well as sequencing of the CDR3 region of both chains.

Retain Labile PTMs

Instrument: Agilent 6545XT AdvanceBio LC/Q-TOF

Summary: Localizing glycosite by LC-MS/MS ECD of infliximab LysC digest

Greater Sequence Coverage

Instrument: Thermo Scientific Q Exactive Orbitrap

Summary: 89% sequence coverage from direct infusion of denatured carbonic.