Thanks to everyone who joined us for our first ever ExD for Thermo Scientific Q Exactive Orbitrap MS user meeting. Especially to our speakers, for the time and effort put into delivering fantastic presentations, and to our co-host, Zef Scientific!
Talks covered a range of topics, including the application of ExD on the UHMR platform for de novo antibody sequencing, membrane protein characterization, and advancing native methods for structural characterization with hybrid ExD-SID technology. Thanks to an expert, engaged audience, we enjoyed an active discussion of what technological and software advancements are most needed in the fields of top-down and native mass spectrometry.
Joseph Meeuwsen from e-MSion demonstrated targeted, top-down analysis with our ExDViewer software, fielding good questions and suggestions for improvement from the group. ExDViewer is available in beta – contact betatesting@e-msion.com for access.
If you were unable to attend, check out the presentation recordings and slides below!
— The teams at e-MSion & Zef Scientific
ExDViewer Demo File: Native Carbonic Anhydrase
Summary: 57% sequence coverage of native carbonic anhydrase using EcHCD (ECD with 40 eV/q of HCD collision energy added).
ExDViewer Demo File: Native NISTmAb Light Chain
Summary: Native, top-down characterization of intact NISTmAb. ECD yields disulfide bond cleavage to liberate the light chain as well as sequencing of CDR3 regions.
Multiple Methods for Dissociation and their Application on the UHMR
Amanda Helms
Brodbelt Lab
University of Texas at Austin
Native Top-Down Analysis of Membrane Proteins Directly from the Lipid Bilayer
Wonhyeuk Jung, Ph.D.
Gupta Lab
Yale School of Medicine
The Application of ECD for De Novo Sequencing of Antibodies
Kelly Gallagher, Ph.D.
Heck Lab
Utrecht University
Electron-Based Dissociation Reveals Sequence and Structure Information for Native Proteins and Protein Complexes
Carter Lantz
Loo Lab
University of California Los Angeles
A Hybrid ECD-SID Device Enhances Top-Down and Structural Characterization of Native Protein Complexes
Chen Du
Wysocki Lab
The Ohio State University
ExD Viewer Demo File: Native Carbonic Anhydrase
Summary: 57% sequence coverage of native carbonic anhydrase using EcHCD (ECD with 40 eV/q of HCD collision energy added).
ExD Viewer Demo File: Native NISTmAb Light Chain
Summary: Native, top-down characterization of intact NISTmAb. ECD yields disulfide bond cleavage to liberate the light chain as well as sequencing of CDR3 regions.
Multiple Methods for Dissociation and their Application on the UHMR
Amanda Helms
Brodbelt Lab
University of Texas at Austin
Native Top-Down Analysis of Membrane Proteins Directly from the Lipid Bilayer
Wonhyeuk Jung, Ph.D.
Gupta Lab
Yale School of Medicine
The Application of ECD for De Novo Sequencing of Antibodies
Kelly Gallagher, Ph.D.
Heck Lab
Utrecht University
Electron-Based Dissociation Reveals Sequence and Structure Information for Native Proteins and Protein Complexes
Carter Lantz
Loo Lab
University of California Los Angeles
A Hybrid ECD-SID Device Enhances Top-Down and Structural Characterization of Native Protein Complexes
Chen Du
Wysocki Lab
The Ohio State University
Electron-based fragmentation now available for the Thermo Scientific Q Exactive Orbitrap family.
