On March 24, 2020, Dr. Liangliang Sun and Ph.D. candidate Xiaojing Shen from the Department of Chemistry at Michigan State University shared their research on the separation and top-down characterization of proteoforms and protein complexes, both native and denatured, using capillary electrophoresis-mass spectrometry (CEMS) and a combination of ECD and CID (“ECciD”).
Since December 2019, Dr. Sun has been an early adopter of e-MSion ExD technology. Here, Sun and Shen discuss using an e-MSion ExD cell on an Agilent 6545XT AdvanceBioTM LC/Q-TOF to obtain extensive sequence coverage of proteins smaller than 30 kDa following separation by CE. They also describe how predicting the electrophoretic mobility of proteoforms can be used to increase confidence of proteoform IDs.
To watch the webinar, click here.
The Sun group also recently presented their work with CEMS and ECD at the 68th annual ASMS conference. Check out Xiaojing Shen’s poster: Improving CZE-MS/MS for both denatured and native top-down proteomics (ThP 544).
ASMS 2020 Reboot conference attendees can view all abstracts, posters, and oral presentations through the online portal until August 31st. Afterwards, members can find conference proceedings through the ASMS website.