ASMS 2022
ECD for the Masses Luncheon
ASMS 2022
ECD for the Masses Luncheon

Thanks to everyone who joined us for our first ever ExD for Thermo Scientific Q Exactive Orbitrap MS user meeting. Especially to our speakers, for the time and effort put into delivering fantastic presentations, and to our co-host, Zef Scientific!

Talks covered a range of topics, including the application of ExD on the UHMR platform for de novo antibody sequencing, membrane protein characterization, and advancing native methods for structural characterization with hybrid ExD-SID technology. Thanks to an expert, engaged audience, we enjoyed an active discussion of what technological and software advancements are most needed in the fields of top-down and native mass spectrometry.

Joseph Meeuwsen from e-MSion demonstrated targeted, top-down analysis with our ExD Viewer software, fielding good questions and suggestions for improvement from the group. ExD Viewer is available in beta – contact betatesting@e-msion.com for access.

If you were unable to attend, check out the presentation recordings and slides below!

— The teams at e-MSion & Zef Scientific

Introduction
ExD Viewer Demo

Introduction

Jared Shaw, Ph.D.
e-MSion

ExD Viewer Demo

Joseph Meeuwsen, Ph.D.
e-MSion

ExD Viewer Demo File: Native Carbonic Anhydrase

Instrument: Thermo Scientific Q Exactive Orbitrap
Summary: 57% sequence coverage of native carbonic anhydrase using EcHCD (ECD with 40 eV/q of HCD collision energy added).

ExD Viewer Demo File: Native NISTmAb Light Chain

Instrument: Thermo Scientific Q Exactive Orbitrap
Summary: Native, top-down characterization of intact NISTmAb. ECD yields disulfide bond cleavage to liberate the light chain as well as sequencing of CDR3 regions.
Lightning Talks

Multiple Methods for Dissociation and their Application on the UHMR

Amanda Helms
Brodbelt Lab
University of Texas at Austin

Native Top-Down Analysis of Membrane Proteins Directly from the Lipid Bilayer

Wonhyeuk Jung, Ph.D.
Gupta Lab
Yale School of Medicine

The Application of ECD for De Novo Sequencing of Antibodies

Kelly Gallagher, Ph.D.
Heck Lab
Utrecht University

Electron-Based Dissociation Reveals Sequence and Structure Information for Native Proteins and Protein Complexes

Carter Lantz
Loo Lab
University of California Los Angeles

A Hybrid ECD-SID Device Enhances Top-Down and Structural Characterization of Native Protein Complexes

Chen Du
Wysocki Lab
The Ohio State University

Introduction

Introduction

Jared Shaw, Ph.D.
e-MSion
ExD Viewer Demo

ExD Viewer Demo

Joseph Meeuwsen, Ph.D.
e-MSion

ExD Viewer Demo File: Native Carbonic Anhydrase

Instrument: Thermo Scientific Q Exactive Orbitrap
Summary: 57% sequence coverage of native carbonic anhydrase using EcHCD (ECD with 40 eV/q of HCD collision energy added).

ExD Viewer Demo File: Native NISTmAb Light Chain

Instrument: Thermo Scientific Q Exactive Orbitrap
Summary: Native, top-down characterization of intact NISTmAb. ECD yields disulfide bond cleavage to liberate the light chain as well as sequencing of CDR3 regions.
Lightning Talks

Multiple Methods for Dissociation and their Application on the UHMR

Amanda Helms
Brodbelt Lab
University of Texas at Austin

Native Top-Down Analysis of Membrane Proteins Directly from the Lipid Bilayer

Wonhyeuk Jung, Ph.D.
Gupta Lab
Yale School of Medicine

The Application of ECD for De Novo Sequencing of Antibodies

Kelly Gallagher, Ph.D.
Heck Lab
Utrecht University

Electron-Based Dissociation Reveals Sequence and Structure Information for Native Proteins and Protein Complexes

Carter Lantz
Loo Lab
University of California Los Angeles

A Hybrid ECD-SID Device Enhances Top-Down and Structural Characterization of Native Protein Complexes

Chen Du
Wysocki Lab
The Ohio State University

About the ExD TQ-160 Option

Electron-based fragmentation now available for the Thermo Scientific Q Exactive Orbitrap family.