Top-Down Protein Sequencing

The following spectrum of the 7th charge state of Ubiquitin was obtained on a Bruker Q-ToF with the ECD cell.  

ECD is particularly efficient with multiply charged proteins compared to doubly charged peptides.  Essentially, complete coverage of ubiquitin from both the Amino-Start and the Carboxy-Terminus as c and z fragments respectively.  The spectra was recorded from an infusion of ubiquitin over 10 minutes.  

ubiquitin.001